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Intranigral transplants of immortalized GABAergic cells decrease the expression of kainic acid-induced seizures in the rat.

Claudia G Castillo·Soledad Mendoza·William J Freed·Magda Giordano
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Abstract

Repeated systemic administration of low doses of kainic acid (KA) induces spontaneous convulsive seizures [Hellier JL, Patrylo PR, Buckmaster PS, Dudek FE. Recurrent spontaneous motor seizures after repeated low-dose systemic treatment with kainate: assessment of a rat model of temporal lobe epilepsy. Epilepsy Res 1998;31:73-84]. In this study, male Sprague-Dawley animals received intranigral transplants of a control cell line M213-2O, or a cell line transfected with human GAD67 cDNA (M213-2O CL4) [Conejero-Goldberg C, Tornatore C, Abi-Saab W, Monaco MC, Dillon-Carter O, Vawter M, et al. Transduction of human GAD67 cDNA into immortalized striatal cell lines using an Epstein-Barr virus-based plasmid vector increases GABA content. Exp Neurol 2000;161:453-61], or no transplant. Eight weeks after transplantation surgery, KA was administered (5 mg/kg/h) until animals reached stage V seizures as described by Racine [Racine RJ. Modification of seizure activity by electrical stimulation. II. Motor seizure. Electroencephalogr Clin Neurophysiol 1972;32:281-94]. The group transplanted with CL4 required a larger dose of KA and a longer latency to reach a stage V seizure. In addition, this group exhibited significantly fewer stage III and IV seizures. These results indicate that intranigral transplants of a GABA-producing cell line can decrease the number of kainic acid-induced seizures.

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AnimalsCell Line, TransformedGlutamate DecarboxylaseHumansIsoenzymesKainic AcidMaleNeostriatumNeuronsRatsRats, Sprague-DawleySeizuresSubstantia NigraTransgenesgamma-Aminobutyric AcidComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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Claudia G Castillo, Soledad Mendoza, William J Freed, Magda Giordano. 2006-05-04. Intranigral transplants of immortalized GABAergic cells decrease the expression of kainic acid-induced seizures in the rat.. https://doi.org/10.1016/j.bbr.2006.03.025

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