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Platelet-activating factor-induced NF-kappaB activation enhances VEGF expression through a decrease in p53 activity.

Hyun-Mi Ko·Hae Hyun Jung·Kook Heon Seo·Yeong-Rim Kang·Han-A Kim·Sung Jun Park·Hern-Ku Lee·Suhn-Young Im
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Abstract

We investigated the role of p53 in nuclear factor (NF)-kappaB dependent, platelet-activating factor (PAF)-induced vascular endothelial growth factor (VEGF) expression. Transfected NF-kappaB subunits in ECV304 cells increased the tumor necrosis factor-alpha promoter activity, which was completely inhibited by p53. Transfected p53 increased p53RE promoter activity, which was completely inhibited by NF-kappaB subunits, indicating that cross-regulation occurs between NF-kappaB and p53. PAF-induced increase in VEGF expression was correlated with decreased p53 activity. These data suggest that NF-kappaB-dependency of the PAF-induced increase in VEGF expression is due to decreased p53 activity, which is reciprocally regulated by increased NF-kappaB activity.

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Cells, CulturedDown-RegulationHumansPlatelet Activating FactorPromoter Regions, GeneticTranscription, GeneticTumor Necrosis Factor-alphaTumor Suppressor Protein p53Vascular Endothelial Growth Factor AJournal ArticleResearch Support, Non-U.S. Gov't

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Hyun-Mi Ko, Hae Hyun Jung, Kook Heon Seo, Yeong-Rim Kang, Han-A Kim, Sung Jun Park, Hern-Ku Lee, Suhn-Young Im. 2006-04-25. Platelet-activating factor-induced NF-kappaB activation enhances VEGF expression through a decrease in p53 activity.. https://doi.org/10.1016/j.febslet.2006.04.042

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