Ribosome dynamics: insights from atomic structure modeling into cryo-electron microscopy maps.

Kakoli Mitra; Joachim Frank

doi:10.1146/annurev.biophys.35.040405.101950

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Ribosome dynamics: insights from atomic structure modeling into cryo-electron microscopy maps.

Kakoli Mitra·Joachim Frank

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Abstract

Single-particle cryo-electron microscopy (cryo-EM) is the method of choice for studying the dynamics of macromolecular machines both at a phenomenological and, increasingly, at the molecular level, with the advent of high-resolution component X-ray structures and of progressively improving fitting algorithms. Cryo-EM has shed light on the structure of the ribosome during the four steps of translation: initiation, elongation, termination, and recycling. Interpretation of cryo-EM reconstructions of the ribosome in quasi-atomic detail reveals a picture in which the ribosome uses RNA not only to catalyze chemical reactions, but also as a means for signal transduction over large distances.

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Computer Simulation Cryoelectron Microscopy Models, Chemical Models, Molecular Motion Protein Conformation Ribosomal Proteins Ribosomes Signal Transduction Structure-Activity Relationship Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.Review

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Kakoli Mitra, Joachim Frank. 2006. Ribosome dynamics: insights from atomic structure modeling into cryo-electron microscopy maps.. https://doi.org/10.1146/annurev.biophys.35.040405.101950

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