Oligo(A) not coded by DNA generating 3'-terminal heterogeneity in a lambda phage RNA.

G R Smith; J Hedgpeth

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PubMed · 167007

Oligo(A) not coded by DNA generating 3'-terminal heterogeneity in a lambda phage RNA.

G R Smith·J Hedgpeth

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Abstract

An RNA species from Escherichia coli infected with phage lambda was purified by hybridization to lambda 1-strand DNA and shown to have variable length. This variability was due to a variable number of adenylate residues attached to the 3' end of the molecule. Pancreatic RNase treatment of the RNA-DNA hybrid removed the 3'-terminal adenylate residues, generating a homogeneous RNA molecule terminating with -Up. The results indicate the presence of adenylate residues not coded by the DNA template at the terminus of this transcript from intact cells.

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G R Smith, J Hedgpeth. 1975-06-25. Oligo(A) not coded by DNA generating 3'-terminal heterogeneity in a lambda phage RNA.. https://pubmed.ncbi.nlm.nih.gov/167007/

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