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PubMed · 16714929

Histologic comparison of autologous fat processing methods.

John G Rose·Mark J Lucarelli·Bradley N Lemke·Richard K Dortzbach·Cynthia A Boxrud·Suzan Obagi·Sarit Patel
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Abstract

PURPOSE: To perform a quantitative analysis of adipocyte viability after fat processing during autologous fat transfer, comparing the processing methods of washing, centrifuging, and sedimentation. METHODS: An experimental study was conducted in which 24 fat samples were obtained after processing from 22 patients undergoing autologous fat transfer. Histologic analysis of periodic acid-Schiff-stained specimens was then performed. RESULTS: Cell counts per high-powered field of intact adipocytes and nucleated adipocytes and adipocyte cross-sectional area were significantly greater in samples processed by sedimentation, compared with those by centrifuging or washing. CONCLUSIONS: Of the various processing techniques currently used during autologous fat transfer, sedimentation appears to yield a higher proportion of viable adipocytes than does washing or centrifuging.

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AdipocytesAdipogenesisAdipose TissueCell CountCell SeparationCell SurvivalCentrifugationFractionation, Field FlowHumansSpecimen HandlingTissue and Organ HarvestingTransplantation, AutologousComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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John G Rose, Mark J Lucarelli, Bradley N Lemke, Richard K Dortzbach, Cynthia A Boxrud, Suzan Obagi, Sarit Patel. Histologic comparison of autologous fat processing methods.. https://doi.org/10.1097/01.iop.0000217710.09941.10

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