Complementary roles for CD2 and LFA-1 adhesion pathways during T cell activation.

P E Moingeon; J L Lucich; C C Stebbins; M A Recny; B P Wallner; S Koyasu; E L Reinherz

doi:10.1002/eji.1830210311

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Complementary roles for CD2 and LFA-1 adhesion pathways during T cell activation.

P E Moingeon·J L Lucich·C C Stebbins·M A Recny·B P Wallner·S Koyasu·E L Reinherz

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Abstract

The influence of T cell receptor (TcR) triggering on T cell adhesion function has been systematically investigated in the present studies; we show that the adhesion function of LFA-1 is minimal in non-activated T cells but is augmented within minutes following TcR-mediated activation. In contrast, CD2 function is essentially optimal in non-activated T cells and undergoes no detectable modification within 12 h of TcR stimulation. Protein kinase C activation augments LFA-1 but not CD2 adhesion function and cyclic AMP reduces LFA-1 adhesion without affecting CD2-LFA-3 interactions. Up-regulation of the LFA-1 pathway occurs in the absence of any detectable surface redistribution of this molecule, suggesting an activation dependent modification leading to a high-affinity ICAM-1 binding state. The TcR independence of CD2 adhesion function implies a critical role of the CD2 pathway in initiating cell-cell interactions prior to TcR engagement and LFA-1-ICAM-1 binding and underscores the complementary nature of the CD2 and LFA-1 adhesion pathways during the immune response.

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Antigens, Differentiation, T-Lymphocyte CD2 Antigens Cell Adhesion Cell Membrane Clone Cells Cytotoxicity, Immunologic Humans In Vitro Techniques Lymphocyte Activation Lymphocyte Function-Associated Antigen-1 Receptors, Antigen, T-Cell Receptors, Immunologic Structure-Activity Relationship T-Lymphocytes Journal Article Research Support, U.S. Gov't, P.H.S.

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P E Moingeon, J L Lucich, C C Stebbins, M A Recny, B P Wallner, S Koyasu, E L Reinherz. 1991. Complementary roles for CD2 and LFA-1 adhesion pathways during T cell activation.. https://doi.org/10.1002/eji.1830210311

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