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DNA microarraying on compact disc surfaces. Application to the analysis of single nucleotide polymorphisms in Plum pox virus.

Sergi Morais·Raquel Marco-Molés·Rosa Puchades·Angel Maquieira
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Abstract

The potential of using compact discs as high throughput screening platforms for DNA microarraying is discussed and applied to discriminate genetic variations of Plum pox virus.

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Compact DisksGenetic VariationOligonucleotide Array Sequence AnalysisPlum Pox VirusPolymorphism, Single NucleotideSensitivity and SpecificitySurface PropertiesJournal Article

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Sergi Morais, Raquel Marco-Molés, Rosa Puchades, Angel Maquieira. 2006-05-02. DNA microarraying on compact disc surfaces. Application to the analysis of single nucleotide polymorphisms in Plum pox virus.. https://doi.org/10.1039/b600049e

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Identification of endothelial proteins by MALDI-MS using a compact disc microfluidic system.

Vascular endothelial proteins have been analyzed using two-dimensional (2D) gel electrophoresis and subsequent mass spectrometry, with separate methods for the intervening sample preparations. Compact disc (CD) technology was found to be rapid, giving high overall yield both with ordinary Coomassie staining and with Sypro Ruby staining. Combined with automatic in-gel digestion, the CD technology has great capacity for large numbers of protein analysis, although for limited sample numbers, manual methods can give similar sequence coverage. In a test set of 48 samples, 45 proteins were identified using the CD preparation technique, 32 identified with higher sequence coverage using the CD technique, 7 with higher using ZipTips in a robotic workstation, and 5 with higher coverage using dried droplets of unpurified samples. In the process of these methodological comparisons, basic patterns for 116 endothelial proteins were defined, representing 297 separate protein spots on the 2D gels.

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