Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy.

Bohumil Maco; Birthe Fahrenkrog; Ning-Ping Huang; Ueli Aebi

doi:10.1007/978-1-59745-000-3_19

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Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy.

Bohumil Maco·Birthe Fahrenkrog·Ning-Ping Huang·Ueli Aebi

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Abstract

To study the ultrastructure of nuclear pore complexes (NPCs), a wide spectrum of different electron microscopy (EM) or atomic force microscopy (AFM) techniques can be employed. The combination of these methods can reveal new insights into the structural and functional organization of this important supramolecular machine through which nucleocytoplasmic transport occurs. Negative staining, quick freezing/freeze-drying/rotary metal shadowing, embedding and thin sectioning, cryoelectron microscopy and tomography, scanning electron microscopy, or combination with immunolabeling techniques are tools for collecting data and information about the three-dimensional structure and architecture of the NPCs. AFM enables investigation of the functional dynamics of native NPCs under physiological conditions.

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Animals Cryoelectron Microscopy Female Microscopy, Atomic Force Microscopy, Electron, Scanning Nuclear Pore Oocytes Structure-Activity Relationship Xenopus laevis Journal Article Research Support, Non-U.S. Gov't Review

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Bohumil Maco, Birthe Fahrenkrog, Ning-Ping Huang, Ueli Aebi. 2006. Nuclear pore complex structure and plasticity revealed by electron and atomic force microscopy.. https://doi.org/10.1007/978-1-59745-000-3_19

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