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PubMed · 16752

The exchange reaction of peptides R-D-alanyl-D-alanine with D-[14C]alanine to R-D-alanyl-D-[14C]alanine and D-alanine, catalysed by the membranes of Streptococcus faecalis ATCC 9790.

J Coyette·J M Ghuysen·H R Perkins
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Abstract

Under alkaline conditions, the membrane-bound DD-carboxypeptidase of Streptococcus faecalis ATCC 9790 catalyses exchange reactions in which the X-L-R3-D-Ala moiety of peptides of the type X-L-R3-D-Ala-D-Ala is transferred to simple amino compounds such as D-alanine, glycine and glycyl-glycine. The enzyme system is unable, however, to catalyse complex reactions that would simulate the natural transpeptidation reaction.

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AlanineCarboxypeptidasesCell MembraneDetergentsEnterococcus faecalisHydrogen-Ion ConcentrationKineticsMuramoylpentapeptide CarboxypeptidaseOligopeptidesOsmolar ConcentrationStructure-Activity RelationshipJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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J Coyette, J M Ghuysen, H R Perkins. 1977-05-02. The exchange reaction of peptides R-D-alanyl-D-alanine with D-[14C]alanine to R-D-alanyl-D-[14C]alanine and D-alanine, catalysed by the membranes of Streptococcus faecalis ATCC 9790.. https://doi.org/10.1111/j.1432-1033.1977.tb11521.x

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PubMed1979

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