Biomolecular sensor based on fluorescence-labeled aptamer.

Hiroaki Ozaki; Akifumi Nishihira; Masayuki Wakabayashi; Masayasu Kuwahara; Hiroaki Sawai

doi:10.1016/j.bmcl.2006.05.054

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Biomolecular sensor based on fluorescence-labeled aptamer.

Hiroaki Ozaki·Akifumi Nishihira·Masayuki Wakabayashi·Masayasu Kuwahara·Hiroaki Sawai

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Abstract

Fluorescent DNA probes for L-argininamide were developed by a combination of DNA aptamers and fluorophore-quencher pairs. These molecules were synthesized by a combination of pre- and post-synthetic modification methods. The fluorescence-labeled aptamer could detect L-argininamide specifically. The binding affinities were defined by the binding affinity of the original aptamer to indicate that the end labeling of the aptamer did not influence the affinities.

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Aptamers, Nucleotide Aptamers, Peptide Arginine Base Sequence Biosensing Techniques Chemistry, Pharmaceutical DNA Drug Design Fluorescent Dyes Ligands Models, Chemical Molecular Sequence Data Spectrometry, Fluorescence Temperature Journal Article

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Hiroaki Ozaki, Akifumi Nishihira, Masayuki Wakabayashi, Masayasu Kuwahara, Hiroaki Sawai. 2006-06-06. Biomolecular sensor based on fluorescence-labeled aptamer.. https://doi.org/10.1016/j.bmcl.2006.05.054

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