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PubMed · 16761723

Transgenic systems in nuclear reprogramming.

Megan Munsie·Peter Mountford·Jennifer Nichols
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Abstract

Transgenic reporters have proved to be invaluable in the study of nuclear reprogramming, from demonstrating revival or silencing of gene expression in fusion hybrids to providing a means to display levels and distribution of specific gene products after nuclear transfer. Here, the method of piezo-assisted direct injection, which has been used previously to generate blastocysts and subsequently embryonic stem cell lines by transfer of nuclei from transgenic reporter mice, is described. This protocol differs from previously described techniques in that the donor nucleus is placed in the recipient oocyte before removal of the host metaphase plate.

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AnimalsBlastocystCell LineCell NucleusCulture MediaEmbryo, MammalianFemaleGenes, ReporterGreen Fluorescent ProteinsLac OperonMiceOocytesOvulationPlasmidsStem CellsTransgenesJournal ArticleResearch Support, Non-U.S. Gov't

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Megan Munsie, Peter Mountford, Jennifer Nichols. 2006. Transgenic systems in nuclear reprogramming.. https://doi.org/10.1385/1-59745-005-7%3A115

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