Process optimization for poly-beta-hydroxybutyrate production in a nitrogen fixing cyanobacterium, Nostoc muscorum using response surface methodology.

Laxuman Sharma; Akhilesh Kumar Singh; Bhabatarini Panda; Nirupama Mallick

doi:10.1016/j.biortech.2006.04.016

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Process optimization for poly-beta-hydroxybutyrate production in a nitrogen fixing cyanobacterium, Nostoc muscorum using response surface methodology.

Laxuman Sharma·Akhilesh Kumar Singh·Bhabatarini Panda·Nirupama Mallick

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Abstract

A five-level-four-factor central composite rotary design was employed to find out the interactive effects of four variables, viz. concentrations of acetate, glucose and K2HPO4, and dark incubation period on poly-beta-hydroxybutyrate (PHB) production in a N2-fixing cyanobacterium, Nostoc muscorum. Acetate, glucose and dark incubation period exhibited positive impacts on PHB yield. Using response surface methodology (RSM), a second order polynomial equation was obtained by multiple regression analysis. A yield of 45.6% of dry cell weight (dcw) was achieved at reduced level of nutrients, i.e. 0.17% acetate, 0.16% glucose and 5 mg l(-1) K2HPO4 at a dark incubation period of 95 h as compared to 41.6% PHB yield in 0.4% acetate, 0.4% glucose and 40 mg l(-1) K2HPO4 at a dark incubation period of 168 h under single factor optimization strategy.

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Acetates Food Glucose Hydroxybutyrates Industrial Microbiology Models, Statistical Nostoc muscorum Phosphates Photoperiod Polyesters Polyhydroxybutyrates Comparative Study Journal Article Research Support, Non-U.S. Gov't

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Laxuman Sharma, Akhilesh Kumar Singh, Bhabatarini Panda, Nirupama Mallick. 2006-06-12. Process optimization for poly-beta-hydroxybutyrate production in a nitrogen fixing cyanobacterium, Nostoc muscorum using response surface methodology.. https://doi.org/10.1016/j.biortech.2006.04.016

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