Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror.

Wibool Piyawattanametha; Robert P J Barretto; Tony H Ko; Benjamin A Flusberg; Eric D Cocker; Hyejun Ra; Daesung Lee; Olav Solgaard; Mark J Schnitzer

doi:10.1364/ol.31.002018

PubMed · 16770418

Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror.

Wibool Piyawattanametha·Robert P J Barretto·Tony H Ko·Benjamin A Flusberg·Eric D Cocker·Hyejun Ra·Daesung Lee·Olav Solgaard·Mark J Schnitzer

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Abstract

Towards overcoming the size limitations of conventional two-photon fluorescence microscopy, we introduce two-photon imaging based on microelectromechanical systems (MEMS) scanners. Single crystalline silicon scanning mirrors that are 0.75 mm x 0.75 mm in size and driven in two dimensions by microfabricated vertical comb electrostatic actuators can provide optical deflection angles through a range of approximately16 degrees . Using such scanners we demonstrated two-photon microscopy and microendoscopy with fast-axis acquisition rates up to 3.52 kHz.

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Computer Systems Equipment Design Equipment Failure Analysis Lenses Mechanics Microscopy, Fluorescence, Multiphoton Miniaturization Reproducibility of Results Sensitivity and Specificity Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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Wibool Piyawattanametha, Robert P J Barretto, Tony H Ko, Benjamin A Flusberg, Eric D Cocker, Hyejun Ra, Daesung Lee, Olav Solgaard, Mark J Schnitzer. 2006-07-01. Fast-scanning two-photon fluorescence imaging based on a microelectromechanical systems two- dimensional scanning mirror.. https://doi.org/10.1364/ol.31.002018

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