Downscaling functional bioassays by single-molecule techniques.

Feng Hong; Douglas D Root

doi:10.1016/j.drudis.2006.05.003

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PubMed · 16793533

Downscaling functional bioassays by single-molecule techniques.

Feng Hong·Douglas D Root

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Abstract

In this short review we examine the potential of single-molecule assays in drug development and in basic research to provide new types of information at the smallest assay scales. A key advantage of many single-molecule assays is the requirement for conservative amounts of precious sample compared to conventional assays. In addition, they measure processes that are not observed directly in molecular ensembles. These advantages are balanced currently by difficulties in assay setup, preparation and equipment expense. However, future developments will ameliorate these drawbacks with the production of simpler, less expensive experimental systems for single-molecule assays.

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Biological Assay Microchemistry Microfluidic Analytical Techniques Microscopy, Atomic Force Microscopy, Fluorescence Nanotechnology Reproducibility of Results Spectrometry, Fluorescence Technology, Pharmaceutical Time Factors Journal Article Review

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Feng Hong, Douglas D Root. 2006. Downscaling functional bioassays by single-molecule techniques.. https://doi.org/10.1016/j.drudis.2006.05.003

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