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PubMed · 1680352

A rapid method for screening vaccinia virus recombinants.

A C Lai·Y Chu
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Abstract

A rapid and small-scale method for screening vaccinia virus recombinants employing micrococcal nuclease is described. This protocol utilizes the differential sensitivity of cellular and viral DNA to the nuclease, which can be selectively activated by addition of Ca2+ and inactivated by elimination of Ca2+. Two to five micrograms of viral DNA can be obtained from one infected L cell plate (50 mm) after overnight incubation.

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AnimalsDNA, RecombinantDNA, ViralElectrophoresis, Agar GelIn Vitro TechniquesL CellsMicePolymorphism, Restriction Fragment LengthVaccinia virusJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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A C Lai, Y Chu. 1991. A rapid method for screening vaccinia virus recombinants.. https://pubmed.ncbi.nlm.nih.gov/1680352/

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