High-resolution analysis of catechol-type siderophores using polyamide thin layer chromatography.

Xiaojun Xie; Jingguo Wang; Hongli Yuan

doi:10.1016/j.mimet.2006.04.022

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High-resolution analysis of catechol-type siderophores using polyamide thin layer chromatography.

Xiaojun Xie·Jingguo Wang·Hongli Yuan

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Abstract

The iron-deficient culture supernatant of a soil bacterial strain identified as Erwinia sp. was analyzed using a new high-resolution polyamide thin layer chromatography (TLC) and a silica TLC. The results showed both TLC methods were very effective for separating simple catechol compounds such as 2,3-dihydroxybenzoic acid (2,3-DHBA) and catechol. However, in the analysis of more complicated catechol compounds or true catechol-type siderophores (conjugates of 2,3-DHBA and amino acids), the polyamide TLC had the higher resolution. Polyamide TLC analysis showed that strain S1 produced three distinct catechol-type siderophores.

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Chromatography, Thin Layer Erwinia Hydroxybenzoates Nylons Siderophores Comparative Study Journal Article Research Support, Non-U.S. Gov't

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Xiaojun Xie, Jingguo Wang, Hongli Yuan. 2006-06-27. High-resolution analysis of catechol-type siderophores using polyamide thin layer chromatography.. https://doi.org/10.1016/j.mimet.2006.04.022

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