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Luminography--a new, highly sensitive visualization method for electrophoresis.

R Schneppenheim·U Budde·N Dahlmann·P Rautenberg
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Abstract

A highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. The method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. The luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. Analogous to autoradiography the luminescent signal is recorded on an X-ray film with similar sensitivity. We present several examples of application emphasizing the general versatility of this innovative method.

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AnimalsAntibodies, HeterophileAutoradiographyBlotting, WesternChromogenic CompoundsDensitometryElectrophoresis, Polyacrylamide GelGliadinHIV AntibodiesHorseradish PeroxidaseHumansHydrogen PeroxideImmunoglobulin GIsoelectric FocusingLuminescent MeasurementsLuminolMiceTransglutaminasesvon Willebrand FactorComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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R Schneppenheim, U Budde, N Dahlmann, P Rautenberg. 1991. Luminography--a new, highly sensitive visualization method for electrophoresis.. https://doi.org/10.1002/elps.1150120508

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