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PubMed · 16825176

[Comparison of methods to construct a full-length cDNA library].

Xin-Guo Mao·Rui-Lian Jing·Xiu-Ying Kong·Guang-Yao Zhao·Ji-Zeng Jia
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Abstract

The use of full-length cDNA libraries is an effective tool to obtain complete gene information in a high-efficiency, high-throughput manner, especially in organisms with huge genomes that are not amenable to whole genome sequencing. In this review, we outlined several methods of full-length cDNA library construction and compared their advantages and disadvantages based on their respective principles. Drawing on our own experience, we described the Cap-trapper method in detail, with an emphasis on its application in wheat full-length cDNA library construction as well as the determination of the ratio of full-length cDNA in a library.

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Cloning, MolecularGene LibraryComparative StudyEnglish AbstractJournal ArticleResearch Support, Non-U.S. Gov't

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Xin-Guo Mao, Rui-Lian Jing, Xiu-Ying Kong, Guang-Yao Zhao, Ji-Zeng Jia. 2006. [Comparison of methods to construct a full-length cDNA library].. https://pubmed.ncbi.nlm.nih.gov/16825176/

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