Monoclonal antibody classification based on epitope-binding using differential antigen disruption.

Ergang Shi; Wen Fury; Wentian Li; Warren Mikulka; Thomas Aldrich; Ashique Rafique; Gang Chen; Simon Hoffenberg; Thomas J Daly; Czeslaw Radziejewski

doi:10.1016/j.jim.2006.05.007

PubMed · 16831443

Monoclonal antibody classification based on epitope-binding using differential antigen disruption.

Ergang Shi·Wen Fury·Wentian Li·Warren Mikulka·Thomas Aldrich·Ashique Rafique·Gang Chen·Simon Hoffenberg·Thomas J Daly·Czeslaw Radziejewski

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Abstract

Currently, classifying a population of specific antigen-reactive monoclonal antibodies (mAbs) according to their epitope-binding properties has been limited to competition assays. Such assays are time consuming, labor intensive and restricted to the number of mAbs in the experiment. To overcome this problem, a differential antigen disruption-based antibody profiling procedure was developed. This procedure rapidly classifies specific antigen-reactive mAbs into epitope-related groups by measuring the binding signal of the antibodies to a set of structurally disrupted antigens and then clustering the antibodies according to the similarity of their binding profiles. The clustering results generated by differential antigen disruption showed a significant concordance with those generated by competition experiments. Therefore, differential antigen disruption method opens an opportunity to assess the entire population of antigen-reactive mAbs according to their epitope-binding properties. In doing so, a set of representative antibodies can be drawn to describe the epitope complexity for systematically exploring their functions.

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Animals Antibodies, Monoclonal Antibody Specificity Antigens Binding Sites, Antibody Binding, Competitive Cluster Analysis Epitope Mapping Epitopes Female Humans Mice Mice, Inbred BALB C Journal Article

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Ergang Shi, Wen Fury, Wentian Li, Warren Mikulka, Thomas Aldrich, Ashique Rafique, Gang Chen, Simon Hoffenberg, Thomas J Daly, Czeslaw Radziejewski. 2006-06-27. Monoclonal antibody classification based on epitope-binding using differential antigen disruption.. https://doi.org/10.1016/j.jim.2006.05.007

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