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A unique and universal molecular barcode array.

Sarah E Pierce·Eula L Fung·Daniel F Jaramillo·Angela M Chu·Ronald W Davis·Corey Nislow·Guri Giaever
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Abstract

Molecular barcode arrays allow the analysis of thousands of biological samples in parallel through the use of unique 20-base-pair (bp) DNA tags. Here we present a new barcode array, which is unique among microarrays in that it includes at least five replicates of every tag feature. The use of smaller dispersed replicate features dramatically improves performance versus a single larger feature and allows the correction of previously undetectable hybridization defects.

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Base SequenceDNA ProbesEquipment DesignEquipment Failure AnalysisExpressed Sequence TagsIn Situ Hybridization, FluorescenceMolecular Probe TechniquesMolecular Sequence DataReproducibility of ResultsSensitivity and SpecificitySequence AlignmentSequence Analysis, DNAJournal ArticleResearch Support, N.I.H., Extramural

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Sarah E Pierce, Eula L Fung, Daniel F Jaramillo, Angela M Chu, Ronald W Davis, Corey Nislow, Guri Giaever. 2006. A unique and universal molecular barcode array.. https://doi.org/10.1038/nmeth905

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