Simple and efficient subtractive hybridization screening.

M R Herfort; A T Garber

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PubMed · 1687108

Simple and efficient subtractive hybridization screening.

M R Herfort·A T Garber

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Abstract

A method of subtractive hybridization screening is presented that does not rely on the availability of large amounts of poly(A)+ RNA. The usefulness of this method is demonstrated by the isolation of cDNA clones that are unique or present at enhanced levels in cDNA libraries derived from a psoriatic skin cell line. A very high level of enrichment for rare cDNA sequences is possible as shown by the isolation of cDNA clones present at a frequency of less than one in one hundred thousand (0.001%). This method is particularly useful in experimental systems where the amount of RNA available is limited.

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Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular DNA Humans Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotide Probes Polymorphism, Restriction Fragment Length Journal Article Research Support, Non-U.S. Gov't

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M R Herfort, A T Garber. 1991. Simple and efficient subtractive hybridization screening.. https://pubmed.ncbi.nlm.nih.gov/1687108/

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