Simple SNP typing assay using a base-discriminating fluorescent probe.

Akimitsu Okamoto; Kazuki Tainaka; Yuji Ochi; Keiichiro Kanatani; Isao Saito

doi:10.1039/b515923g

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Simple SNP typing assay using a base-discriminating fluorescent probe.

Akimitsu Okamoto·Kazuki Tainaka·Yuji Ochi·Keiichiro Kanatani·Isao Saito

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Abstract

We have developed a new concept involving a single-step homogeneous method for single-nucleotide polymorphism (SNP) typing. In this method, a probe containing base-discriminating fluorescent (BDF) bases is added to a sample solution. BDF base-containing DNA usually shows only a weak fluorescence, but emits a strong blue fluorescence when it recognizes a target base at a specific site in a hybridized strand. By utilizing this feature, a simple mix-and-read SNP typing assay was achieved without any tedious probe-designing or washing processes for exclusion of hybridization error or any addition of DNA-modifying enzymes. This is very different from conventional methods. We simultaneously analyzed a number of samples with ease, with a high accuracy, using our BDF assay.

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Base Sequence Biological Assay DNA DNA Probes Female Fluorescent Dyes Humans Models, Molecular Nucleic Acid Hybridization Polymerase Chain Reaction Polymorphism, Single Nucleotide Spectrometry, Fluorescence Journal Article

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Akimitsu Okamoto, Kazuki Tainaka, Yuji Ochi, Keiichiro Kanatani, Isao Saito. 2006-01-05. Simple SNP typing assay using a base-discriminating fluorescent probe.. https://doi.org/10.1039/b515923g

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