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A microRNA detection system based on padlock probes and rolling circle amplification.

Søren Peter Jonstrup·Jørn Koch·Jørgen Kjems
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Abstract

The differential expression and the regulatory roles of microRNAs (miRNAs) are being studied intensively these years. Their minute size of only 19-24 nucleotides and strong sequence similarity among related species call for enhanced methods for reliable detection and quantification. Moreover, miRNA expression is generally restricted to a limited number of specific cells within an organism and therefore requires highly sensitive detection methods. Here we present a simple and reliable miRNA detection protocol based on padlock probes and rolling circle amplification. It can be performed without specialized equipment and is capable of measuring the content of specific miRNAs in a few nanograms of total RNA.

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AnimalsBacteriophagesCell LineCell Line, TumorDNA LigasesDNA-Directed DNA PolymeraseHumansLiverMiceMicroRNAsNeuroblastomaNucleic Acid Amplification TechniquesRNARNA ProbesRNA, CircularTemplates, GeneticComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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Søren Peter Jonstrup, Jørn Koch, Jørgen Kjems. 2006-08-03. A microRNA detection system based on padlock probes and rolling circle amplification.. https://doi.org/10.1261/rna.110706

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