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A binary DNA probe for highly specific nucleic Acid recognition.

Dmitry M Kolpashchikov
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Abstract

A new concept for nucleic acid probe design is reported. The extremely high selectivity of the probe is predetermined by cooperative hybridization of the two relatively short (10 nucleotide) DNA hairpin fragments to the analyte. A binary DNA probe fluorescently reports the presence of 0.5% of the analyte in excess amount of a single base substituted oligodeoxyribonucleotide and distinguishes single nucleotide substitutions at any position of a 20-mer oligonucleotide at room temperature.

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Base SequenceDNAFluorescent DyesMolecular Sequence DataNucleic Acid ConformationNucleic AcidsSensitivity and SpecificityJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, U.S. Gov't, Non-P.H.S.

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Dmitry M Kolpashchikov. 2006-08-16. A binary DNA probe for highly specific nucleic Acid recognition.. https://doi.org/10.1021/ja0628093

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