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Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).

Michael J Rust·Mark Bates·Xiaowei Zhuang
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Abstract

We have developed a high-resolution fluorescence microscopy method based on high-accuracy localization of photoswitchable fluorophores. In each imaging cycle, only a fraction of the fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were used to reconstruct the overall image. We demonstrated an imaging resolution of 20 nm. This technique can, in principle, reach molecular-scale resolution.

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DNAFluorescent DyesImage Interpretation, Computer-AssistedMicroscopy, FluorescenceNanotechnologyStochastic ProcessesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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Michael J Rust, Mark Bates, Xiaowei Zhuang. 2006-08-09. Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (STORM).. https://doi.org/10.1038/nmeth929

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