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Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: intracellular concentration decrease in NAD and increase in cyclic ADP-ribose.

Haruhiro Higashida·Alla Salmina·Minako Hashii·Shigeru Yokoyama·Jia-Sheng Zhang·Mami Noda·Zen-Guo Zhong·Duo Jin
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Abstract

ADP-ribosyl cyclase activity in the crude membrane fraction of neuroblastomaxglioma NGPM1-27 hybrid cells was measured by monitoring [(3)H] cyclic ADP-ribose (cADPR) formation from [(3)H] NAD(+). Bradykinin (BK) at 100nM increased ADP-ribosyl cyclase activity by about 2.5-fold. Application of 300nM BK to living NGPM1-27 cells decreased NAD(+) to 78% of the prestimulation level at 30s. In contrast, intracellular cADPR concentrations were increased by 2-3-fold during the period from 30 to 120s after the same treatment. Our results suggest that cADPR is one of the second messengers downstream of B(2) BK receptors.

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ADP-ribosyl CyclaseAnimalsBradykininCell Line, TumorCell MembraneCyclic ADP-RiboseHybrid CellsMiceNADNeuroblastomaRatsSecond Messenger SystemsVasodilator AgentsJournal Article

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Haruhiro Higashida, Alla Salmina, Minako Hashii, Shigeru Yokoyama, Jia-Sheng Zhang, Mami Noda, Zen-Guo Zhong, Duo Jin. 2006-08-08. Bradykinin activates ADP-ribosyl cyclase in neuroblastoma cells: intracellular concentration decrease in NAD and increase in cyclic ADP-ribose.. https://doi.org/10.1016/j.febslet.2006.07.084

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