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Sulphate and osteoarthritis: decrease of serum sulphate levels by an additional 3-h fast and a 3-h glucose tolerance test after an overnight fast.

C M Blinn·B A Biggee·T E McAlindon·M Nuite·J E Silbert
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Abstract

BACKGROUND: Low sulphate levels in blood may contribute to osteoarthritis by decreasing cartilage chondroitin sulphation. OBJECTIVE: To measure serum levels of sulphate during 3 h of fasting or glucose ingestion after overnight fasts to determine how much sulphate lowering may occur during this period. METHODS: Sera from 14 patients with osteoarthritis who fasted overnight were obtained every 15-30 min during 3 h of continued fasting and during 3 h after ingestion of 75 g of glucose. Sulphate was assayed by high-performance liquid chromatography with a Metrohm-Peak 761 Compact IC and simultaneously assayed for glucose by high-performance liquid chromatography with a Metrohm-Peak 817 Bioscan. RESULTS: Continuation of overnight fasting for 3 h resulted in a near-linear 3-h decrease in levels for all 14 patients ranging from 3% to 20% with a mean drop of 9.3%, whereas the 3-h decrease after glucose ingestion ranged from 10% to 33% with a mean drop of 18.9%. CONCLUSION: A 3-h continuation of fasting caused a marked reduction in serum sulphate levels, whereas ingestion of 75 g of glucose in the absence of protein resulted in doubling the reduction. This suggests that fasting and ingestion of protein-free calories may produce periods of chondroitin undersulphation that could affect osteoarthritis.

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AdultAgedBlood GlucoseChromatography, High Pressure LiquidFastingFemaleGlucose Tolerance TestHumansMaleMiddle AgedOsteoarthritisSulfatesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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C M Blinn, B A Biggee, T E McAlindon, M Nuite, J E Silbert. 2006. Sulphate and osteoarthritis: decrease of serum sulphate levels by an additional 3-h fast and a 3-h glucose tolerance test after an overnight fast.. https://doi.org/10.1136/ard.2006.052571

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