New embedding medium for sectioning undecalcified bone.

S Mohsin; F J O' Brien; T C Lee

doi:10.1080/10520290600834136

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PubMed · 16908434

New embedding medium for sectioning undecalcified bone.

S Mohsin·F J O' Brien·T C Lee

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Abstract

Methylmethacrylate (MMA) is the most commonly used embedding medium for sectioning undecalcified bone; however, a number of problems exist with its use in a research laboratory. MMA requires a long infiltration time and temperature control, and it reacts with many polymers. We used Kleer Set resin as an alternative embedding medium for sectioning undecalcified bone specimens. Fluorochrome labeled bone specimens were sectioned transversely using a ground section technique and longitudinally on a sledge macrotome. The slides were viewed using both transmitted light and epifluorescence microscopy. High quality sections were obtained using Kleer Set resin for both sectioning techniques. We have shown that this new embedding medium is simpler, safer, quicker to use and does not interfere with visualization of fluorochromes.

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Animals Bone and Bones Microscopy, Fluorescence Microtomy Resins, Synthetic Sheep Tissue Embedding Journal Article Research Support, Non-U.S. Gov't

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S Mohsin, F J O' Brien, T C Lee. New embedding medium for sectioning undecalcified bone.. https://doi.org/10.1080/10520290600834136

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