Reovirus structure and morphogenesis.

K M Coombs

doi:10.1007/3-540-30773-7_5

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Reovirus structure and morphogenesis.

K M Coombs

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Abstract

Assembly of a mature infectious virion from component parts is one of the last steps in the replicative cycle of most viruses. Recent advances in delineating aspects of this process for the mammalian orthoreoviruses (MRV), nonenveloped viruses composed of a genome of ten segments of double-stranded RNA enclosed in two concentric icosahedral protein capsids, are discussed. Analyses of temperature-sensitive (ts) assembly-defective reovirus mutants have been used to better understand requirements for viral inclusion formation and capsid morphogenesis. Newly determined high-resolution structures of virtually all MRV proteins, combined with complete MRV genomic sequence information and elucidation of sequence lesions in ts mutants, is now providing a context for molecularly understanding interactions that promote, or abrogate, reovirus capsid assembly. Additional advances in understanding required signals for whole genome construction from sets of the ten individual genes, and in transcapsidation of subviral particles with engineered outer capsid proteins, provide additional molecular genetic understanding of reovirus protein structure-function and morphogenesis.

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Capsid Genome, Viral Morphogenesis Reoviridae Temperature Virus Assembly Virus Replication Journal Article Research Support, Non-U.S. Gov't

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K M Coombs. 2006. Reovirus structure and morphogenesis.. https://doi.org/10.1007/3-540-30773-7_5

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