A solid phase reverse transcriptase micro-assay for the detection of human immunodeficiency virus and other retroviruses in cell culture supernatants.

P A Somogyi; A Gyuris; I Földes

doi:10.1016/0166-0934(90)90095-w

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A solid phase reverse transcriptase micro-assay for the detection of human immunodeficiency virus and other retroviruses in cell culture supernatants.

P A Somogyi·A Gyuris·I Földes

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Abstract

A simple and rapid solid-phase reverse transcriptase assay was developed based on the use of poly(rA):oligo(dT)12-18 as template primer immobilized on DEAE cellulose paper squares to detect human immunodeficiency virus (HIV) and/or other retroviruses in cell culture supernatants. It was found that PEG (per se) -up to 4% concentrations (w/v)--did not inhibit reverse transcriptase activity. Optimal conditions of the assay were determined. This solid-phase technique is much faster and more convenient than the methods described previously.

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Cells, Cultured Chemical Precipitation HIV Poly A Poly T Poly dA-dT Polyethylene Glycols RNA-Directed DNA Polymerase Retroviridae Journal Article

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P A Somogyi, A Gyuris, I Földes. 1990. A solid phase reverse transcriptase micro-assay for the detection of human immunodeficiency virus and other retroviruses in cell culture supernatants.. https://doi.org/10.1016/0166-0934(90)90095-w

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