Three-dimensional tissue constructs built by bioprinting.

Karoly Jakab; Brook Damon; Adrian Neagu; Anatolij Kachurin; Gabor Forgacs

PubMed · 16912422

Three-dimensional tissue constructs built by bioprinting.

Karoly Jakab·Brook Damon·Adrian Neagu·Anatolij Kachurin·Gabor Forgacs

Abstract

Bioprinting is an evolving tissue engineering technology. It utilizes computer controlled three-dimensional printers for rapid and high-precision construction of three-dimensional biological structures. We employed discrete and continuous bioprinting to build three-dimensional tissue constructs. In the former case bioink particles - spherical cell aggregates composed of many thousands of cells - are delivered one by one into biocompatible scaffolds, the biopaper. Structure formation takes place by the subsequent fusion of the bioink particles due to their liquid-like and self-assembly properties. In the latter case a mixture of cells and scaffold material is extruded from the biocartridge akin to toothpaste to arrive at the desired construct. Specifically, we built rectangular tissue blocks of several hundred microns in thickness as well as tubular structures of several millimeters in height. The physical basis of structure formation was studied by computer simulations.

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Animals CHO Cells Cell Aggregation Computer Peripherals Computer Simulation Cricetinae Cricetulus Models, Biological Spheroids, Cellular Tissue Engineering Evaluation Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

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Karoly Jakab, Brook Damon, Adrian Neagu, Anatolij Kachurin, Gabor Forgacs. Three-dimensional tissue constructs built by bioprinting.. https://pubmed.ncbi.nlm.nih.gov/16912422/

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