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Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis.

Guijie Zhu·Huiming Yuan·Peng Zhao·Lihua Zhang·Zhen Liang·Weibing Zhang·Yukui Zhang
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Abstract

Monolithic materials were prepared in capillaries by in situ polymerization of acrylamide, glycidyl methacrylate, and N,N'-methylenebisacrylamide in the presence of 1,4-butanediol, dodecanol, and DMSO as porogens. With Ampholine attached to the surface of the porous monolith via epoxide groups, a monolithic-IPG (M-IPG) was formed and showed good mechanical and chemical stability. With such a column immobilized by Ampholine 3.5-10, IEF-MIX 3.6-9.3 was separated and good linearity was obtained. The CIEF behavior of M-IPG was evinced by comparing the current with that in the open tubular capillary. In addition, the protein mixtures excreted from lung cancer cells of rats were analyzed with such a new M-IPG column.

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Acrylic ResinsButylene GlycolsDimethyl SulfoxideDodecanolElectrophoresis, CapillaryHumansHydrogen-Ion ConcentrationPorosityProteinsReproducibility of ResultsPolyacrylamidesJournal ArticleResearch Support, Non-U.S. Gov't

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Guijie Zhu, Huiming Yuan, Peng Zhao, Lihua Zhang, Zhen Liang, Weibing Zhang, Yukui Zhang. 2006. Macroporous polyacrylamide-based monolithic column with immobilized pH gradient for protein analysis.. https://doi.org/10.1002/elps.200600189

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