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PubMed · 1692359

[A method of separating mononuclears on a density gradient].

A S Prilutskiĭ·A V Khodakovskiĭ·E A Maĭlian
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Abstract

The developed procedure for the isolation of mononuclears in density gradient d = 1.0077 g/cm3 consists in separation of the plasma by preliminary centrifugation at 200 g for 7-10 min and isolation of leukocytic mononuclear cells from the cellular sediment diluted with the isotonic solution. The length of centrifugation at 400 g is selected with consideration for the thickness of the density gradient. An anticoagulant is added to the isotonic solution and ficoll-verografin mixture to improve the quality of the cell isolation.

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Cell SeparationCentrifugation, Density GradientHumansLeukocytes, MononuclearEnglish AbstractJournal Article

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A S Prilutskiĭ, A V Khodakovskiĭ, E A Maĭlian. 1990. [A method of separating mononuclears on a density gradient].. https://pubmed.ncbi.nlm.nih.gov/1692359/

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