A chemical toolkit for proteins--an expanded genetic code.

Jianming Xie; Peter G Schultz

doi:10.1038/nrm2005

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A chemical toolkit for proteins--an expanded genetic code.

Jianming Xie·Peter G Schultz

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Abstract

Recently, a method to encode unnatural amino acids with diverse physicochemical and biological properties genetically in bacteria, yeast and mammalian cells was developed. Over 30 unnatural amino acids have been co-translationally incorporated into proteins with high fidelity and efficiency using a unique codon and corresponding transfer-RNA:aminoacyl-tRNA-synthetase pair. This provides a powerful tool for exploring protein structure and function in vitro and in vivo, and for generating proteins with new or enhanced properties.

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Amino Acids Amino Acyl-tRNA Synthetases Base Sequence Codon Escherichia coli Genetic Code In Vitro Techniques Molecular Sequence Data Mutagenesis, Site-Directed Mutation Protein Biosynthesis Protein Engineering Proteins RNA, Transfer Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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Jianming Xie, Peter G Schultz. 2006-08-23. A chemical toolkit for proteins--an expanded genetic code.. https://doi.org/10.1038/nrm2005

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