Enzymatically prepared RNAi libraries.

Frank Buchholz; Ralf Kittler; Mikolaj Slabicki; Mirko Theis

doi:10.1038/nmeth912

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Enzymatically prepared RNAi libraries.

Frank Buchholz·Ralf Kittler·Mikolaj Slabicki·Mirko Theis

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Abstract

Large-scale RNA interference (RNAi) screens in mammalian cells have mainly used synthetic small interfering RNA (siRNA) or short hairpin RNA (shRNA) libraries. The RNAi triggers for both of these approaches were designed with algorithm-based predictions to identify single sequences for mRNA knockdown. Alternatives to these approaches have recently been developed using enzymatic methods. Here we describe the concepts of enzymatically prepared shRNA and siRNA libraries, and discuss their strengths and limitations.

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Animals DNA, Complementary DNA-Binding Proteins Gene Library Genetic Vectors Humans RNA Interference RNA, Small Interfering Journal Article Research Support, Non-U.S. Gov't Review

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Frank Buchholz, Ralf Kittler, Mikolaj Slabicki, Mirko Theis. 2006. Enzymatically prepared RNAi libraries.. https://doi.org/10.1038/nmeth912

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