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Digital quantification using amplified single-molecule detection.

Jonas Jarvius·Jonas Melin·Jenny Göransson·Johan Stenberg·Simon Fredriksson·Carlos Gonzalez-Rey·Stefan Bertilsson·Mats Nilsson
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Abstract

We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.

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DNA, BacterialDNA, CircularMolecular Probe TechniquesNanotechnologySensitivity and SpecificitySpectrometry, FluorescenceVibrio choleraeJournal ArticleResearch Support, Non-U.S. Gov't

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Jonas Jarvius, Jonas Melin, Jenny Göransson, Johan Stenberg, Simon Fredriksson, Carlos Gonzalez-Rey, Stefan Bertilsson, Mats Nilsson. 2006. Digital quantification using amplified single-molecule detection.. https://doi.org/10.1038/nmeth916

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