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Recirculating flow accelerates DNA microarray hybridization in a microfluidic device.

Hyun Ho Lee·James Smoot·Zack McMurray·David A Stahl·Paul Yager
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Abstract

A recirculating microfluidic device fabricated by laminating Mylar and glass was developed for the analysis of hybridization of oligonucleotides to DNA microarrays. The device is part of a system that provides controlled hybridization to DNA probes immobilized in a microarray of polyacrylamide gel pads using recirculation and temperature control. The system was used to obtain real-time kinetics of DNA hybridization and more accurate melting profiles of target-probe duplexes than possible using a static hybridization format. Recirculation shortened the time of perfect match target-probe hybridization from 6 hours to 2 hours and shifted the Td by 1.54 degrees C, relative to static conditions. The experimental results were consistent with a three-dimensional simulation of hybridization using a recirculating buffer system.

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BuffersComputer SimulationDNAGelsKineticsMicrofluidic Analytical TechniquesNucleic Acid HybridizationOligonucleotide Array Sequence AnalysisOligonucleotidesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, U.S. Gov't, Non-P.H.S.

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Hyun Ho Lee, James Smoot, Zack McMurray, David A Stahl, Paul Yager. 2006-07-13. Recirculating flow accelerates DNA microarray hybridization in a microfluidic device.. https://doi.org/10.1039/b605507a

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