Actinomycin D binding in vitro: active chromatin preferred.

F L Yu; W Bender

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PubMed · 1693849

Actinomycin D binding in vitro: active chromatin preferred.

F L Yu·W Bender

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Abstract

When [3H] Actinomycin D (Act. D) is used to interact with nuclei and nucleoli in vitro, it binds preferentially to nucleolar chromatin. The preferential binding is no longer detectable, when purified nuclear and nucleolar DNAs are used. In parallel, Act. D preferentially inhibits nucleolar over nuclear RNA synthesis when chromatin templates are used, and the preferential inhibition is lost when purified nuclear and nucleolar DNAs are used. It is concluded: 1) the preferential inhibition of nucleolar over nuclear RNA synthesis by Act. D is a direct reflection of the preferential binding of Act. D to the nucleolar chromatin; and 2) the nucleolar chromosomal proteins, not the nucleolar DNA, confer the preferential binding of Act. D.

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Animals Cell Nucleolus Cell Nucleus Chromatin Chromatography, Ion Exchange DNA DNA-Directed RNA Polymerases Dactinomycin Liver RNA Rats Transcription, Genetic Journal Article Research Support, U.S. Gov't, P.H.S.

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F L Yu, W Bender. 1990. Actinomycin D binding in vitro: active chromatin preferred.. https://pubmed.ncbi.nlm.nih.gov/1693849/

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