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RNA extraction for arrays.

Lakshmi V Madabusi·Gary J Latham·Bernard F Andruss
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Abstract

DNA microarrays enable insights into global gene expression by capturing a snapshot of cellular expression levels at the time of sample collection. Careful RNA handling and extraction are required to preserve this information properly, ensure sample-to-sample reproducibility, and limit unwanted technical variation in experimental data. This chapter discusses important considerations for "array-friendly" sample handling and processing from biosamples such as blood, formalin-fixed, paraffin-embedded samples, and fresh or flash-frozen tissues and cells. It also provides guidelines on RNA quality assessments, which can be used to validate sample preparation and maximize recovery of relevant biological information.

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Lakshmi V Madabusi, Gary J Latham, Bernard F Andruss. 2006. RNA extraction for arrays.. https://doi.org/10.1016/s0076-6879(06)11001-0

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