Protein sulfation analysis--A primer.

Flavio Monigatti; Brian Hekking; Hanno Steen

doi:10.1016/j.bbapap.2006.07.002

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Protein sulfation analysis--A primer.

Flavio Monigatti·Brian Hekking·Hanno Steen

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Abstract

The aim of this review is to present an overview of protein sulfation in the context of 'modificomics', i.e. post-translational modification-specific proteome research. In addition to a short introduction to the biology of protein sulfation (part 1), we will provide detailed discussion regarding (i) methods and tools for prediction of protein tyrosine sulfation sites (part 2), (ii) biochemical techniques used for protein sulfation analysis (part 3.1), and (iii) mass spectrometric strategies and methods applied to protein sulfation analysis (part 3.2). We will highlight strengths and limitations of different strategies and approaches (including references), providing a primer for newcomers to protein sulfation analysis.

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Animals Electron Transport Factor VIII Gas Chromatography-Mass Spectrometry Humans Mass Spectrometry Membrane Glycoproteins Protein Processing, Post-Translational Proteins Receptors, CCR5 Sequence Analysis, Protein Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Sulfotransferases Tyrosine von Willebrand Factor Journal Article Review

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Flavio Monigatti, Brian Hekking, Hanno Steen. 2006-07-26. Protein sulfation analysis--A primer.. https://doi.org/10.1016/j.bbapap.2006.07.002

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