Z-DNA affinity chromatography.

R Fishel; P Anziano; A Rich

doi:10.1016/0076-6879(90)84290-w

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Z-DNA affinity chromatography.

R Fishel·P Anziano·A Rich

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Abstract

In this chapter we have detailed a method that can be generalized to link virtually any DNA substrate to a chromatography matrix at its ends via an avidin-biotin linkage. We have used this technique to construct a left-handed Z-DNA column for the purpose of identification and purification of Z-DNA-binding proteins. This technique for the linkage of DNA to a column matrix by avidin-biotin technology can be modified, however, to produce linked multimeric sequences specific for regulatory or other DNA-binding proteins.

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Avian Myeloblastosis Virus Biotin Chromatography, Affinity Circular Dichroism DNA DNA-Directed DNA Polymerase Electrophoresis, Polyacrylamide Gel Escherichia coli Indicators and Reagents Micrococcus Molecular Weight Nucleic Acid Conformation Polydeoxyribonucleotides RNA-Directed DNA Polymerase Spectrophotometry, Ultraviolet T-Phages Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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R Fishel, P Anziano, A Rich. 1990. Z-DNA affinity chromatography.. https://doi.org/10.1016/0076-6879(90)84290-w

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