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RNAi in Haemonchus contortus: a potential method for target validation.

Jody L Zawadzki·Paul J A Presidente·Els N Meeusen·Michael J De Veer
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Abstract

RNA interference (RNAi) is a method for the functional analysis of specific genes, and is particularly well developed in the free-living nematode Caenorhabditis elegans. There have been several attempts to apply this method to parasitic nematodes. In a recent study undertaken in Haemonchus contortus, Geldhof and colleagues concluded that, although a mechanism for RNAi existed, the methods developed for RNAi in C. elegans had variable efficacy in this parasitic nematode. The potential benefits of RNAi are clear; however, further studies are required to characterize the mechanism present in parasitic nematodes, and to improve culture systems for these nematodes to monitor the long-term effects of RNAi. Only then could RNAi become a reliable assay of gene function.

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AnimalsGene TargetingGenes, HelminthHaemonchusRNA InterferenceRNA, HelminthJournal Article

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Jody L Zawadzki, Paul J A Presidente, Els N Meeusen, Michael J De Veer. 2006-09-12. RNAi in Haemonchus contortus: a potential method for target validation.. https://doi.org/10.1016/j.pt.2006.08.015

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