Single-particle evanescent light scattering simulations for total internal reflection microscopy.

Laurent Helden; Elena Eremina; Norbert Riefler; Christopher Hertlein; Clemens Bechinger; Yuri Eremin; Thomas Wriedt

doi:10.1364/ao.45.007299

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Single-particle evanescent light scattering simulations for total internal reflection microscopy.

Laurent Helden·Elena Eremina·Norbert Riefler·Christopher Hertlein·Clemens Bechinger·Yuri Eremin·Thomas Wriedt

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Abstract

We simulate and measure light scattering of a micrometer-sized spherical particle suspended in solution close to a glass substrate. The model, based on the discrete sources method, is developed to describe the experimental situation of total internal reflection microscopy experiments; i.e., the particle is illuminated by an evanescent light field originating from the glass-solvent interface. In contrast to the well-established assumption of a simple exponential decay of the scattering intensity with distance, we demonstrate significant deviations for a certain range of penetration depths and polarization states of the incident light.

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Laurent Helden, Elena Eremina, Norbert Riefler, Christopher Hertlein, Clemens Bechinger, Yuri Eremin, Thomas Wriedt. 2006-10-01. Single-particle evanescent light scattering simulations for total internal reflection microscopy.. https://doi.org/10.1364/ao.45.007299

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