Medicago truncatula transformation using leaf explants.

Viviane Cosson; Patricia Durand; Isabelle d'Erfurth; Adam Kondorosi; Pascal Ratet

doi:10.1385/1-59745-130-4:115

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Medicago truncatula transformation using leaf explants.

Viviane Cosson·Patricia Durand·Isabelle d'Erfurth·Adam Kondorosi·Pascal Ratet

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Abstract

Legumes have long been recalcitrant to efficient Agrobacterium tumefaciens-mediated transformation. The choice and use of model legume plants (Medicago truncatula and Lotus japonicus) for molecular studies has triggered extensive studies devoted to the development of efficient Agrobacterium-mediated transformation protocols for these two plants. In M. truncatula, transformation protocols rely on the use of highly regenerable lines obtained by recurrent in vitro culture selection. These protocols are based on Agrobacterium-mediated transformation of M. truncatula followed by somatic embryogenesis-mediated plant regeneration. We describe here the protocol developed for M. truncatula R108-1 (c3).

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Agrobacterium tumefaciens Embryonic Development Gene Transfer Techniques Medicago truncatula Plant Leaves Regeneration Transformation, Genetic Journal Article Review

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Viviane Cosson, Patricia Durand, Isabelle d'Erfurth, Adam Kondorosi, Pascal Ratet. 2006. Medicago truncatula transformation using leaf explants.. https://doi.org/10.1385/1-59745-130-4%3A115

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