Medicago truncatula transformation using cotyledon explants.

Elane Wright; Richard A Dixon; Zeng-Yu Wang

doi:10.1385/1-59745-130-4:129

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Medicago truncatula transformation using cotyledon explants.

Elane Wright·Richard A Dixon·Zeng-Yu Wang

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Abstract

Medicago truncatula has been developed into a model species for legumes. Large numbers of ESTs have been sequenced, and sequencing of the complete gene-rich space of the genotype Jemalong A17 is in progress. By using cotyledons as explants for Agrobacterium infection and direct shoot formation, this protocol allows for rapid production of transgenic plants from A17 and other genotypes. Transgenic plants can be regenerated and established in the greenhouse in only 3-4 mo after Agrobacterium-mediated transformation. Transformation frequency is in the range of 5-12%.

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Agrobacterium tumefaciens Cotyledon Gene Transfer Techniques Genotype Medicago truncatula Plant Shoots Plants, Genetically Modified Transformation, Genetic Journal Article Review

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Elane Wright, Richard A Dixon, Zeng-Yu Wang. 2006. Medicago truncatula transformation using cotyledon explants.. https://doi.org/10.1385/1-59745-130-4%3A129

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