C. elegans deletion mutant screening.

Robert J Barstead; Donald G Moerman

doi:10.1385/1-59745-151-7:51

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C. elegans deletion mutant screening.

Robert J Barstead·Donald G Moerman

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Abstract

The methods used by the Caenorhabditis elegans Gene Knockout Consortium are conceptually simple. One does a chemical mutagenesis of wild-type C. elegans, and then screens the progeny of the mutagenized animals, in small mixed groups, using polymerase chain reaction (PCR) to identify populations with animals where a portion of DNA bounded by the PCR primers has been deleted. Animals from such populations are then selected and grown clonally to recover a pure genetic strain. We categorize the steps needed to do this as follows: (1) mutagenesis and DNA template preparation, (2) PCR detection of deletions, (3) sibling selection, and (4) deletion stabilization. These are discussed in detail in this chapter.

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Animals Animals, Genetically Modified Base Sequence Caenorhabditis elegans DNA Mutational Analysis Genes, Helminth Mutagenesis Sequence Deletion Journal Article Review

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Robert J Barstead, Donald G Moerman. 2006. C. elegans deletion mutant screening.. https://doi.org/10.1385/1-59745-151-7%3A51

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