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PubMed · 16999974

New screening method for basic compounds in urine by on-line extraction-high-performance liquid chromatography with photodiode-array detection.

Lena Schönberg·Thomas Grobosch·Dagmar Lampe·Charlotte Kloft
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Abstract

A fully automated, qualitative screening HPLC method for the identification of basic compounds in urine has been developed. A 1-ml volume of urine was extracted by on-line extraction and separated on two coupled strong cation-exchange (SCX) columns (2 x LunaSCX, 150 mm x 4.6 mm, 5 microm) under isocratic conditions. The mobile phase consisted of a mixture of potassium dihydrogenphosphate buffer (pH 2.3) and acetonitrile. The use of photodiode-array detection (DAD, lambda = 190-800 nm) gave access to a library of approximately 2600 toxicologically relevant compounds. The validated method is reliable, simple and in addition successfully proven with the analysis of real biological specimen for the routine use.

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AlkaloidsAmphetaminesChromatography, High Pressure LiquidHumansOnline SystemsOpioid PeptidesReproducibility of ResultsJournal Article

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Lena Schönberg, Thomas Grobosch, Dagmar Lampe, Charlotte Kloft. 2006-09-26. New screening method for basic compounds in urine by on-line extraction-high-performance liquid chromatography with photodiode-array detection.. https://doi.org/10.1016/j.chroma.2006.09.009

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