A bioinformatics pipeline for high-throughput microbial multilocus sequence typing (MLST) analyses.

S Platt; B Pichon; R George; J Green

doi:10.1111/j.1469-0691.2006.01541.x

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A bioinformatics pipeline for high-throughput microbial multilocus sequence typing (MLST) analyses.

S Platt·B Pichon·R George·J Green

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Abstract

Multilocus sequence typing (MLST) analysis for semi-routine applications is hindered by the downstream, manually intensive steps of processing the raw sequence data files. This report describes the development of an MLST pipeline that automates DNA sequence editing and analysis in order to significantly reduce the time required for processing data. Validation using a pneumococcal dataset revealed complete agreement between the results generated by manual and automated workflows. The MLST pipeline was developed for both double-strand and single-strand sequencing.

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Bacteria Bacterial Typing Techniques Cluster Analysis Computational Biology DNA, Bacterial Electronic Data Processing Sequence Analysis Journal Article Research Support, Non-U.S. Gov't

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S Platt, B Pichon, R George, J Green. 2006. A bioinformatics pipeline for high-throughput microbial multilocus sequence typing (MLST) analyses.. https://doi.org/10.1111/j.1469-0691.2006.01541.x

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