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Double-quantum filtered rotational-echo double resonance.

Shigeru Matsuoka·Jacob Schaefer
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Abstract

The homonuclear scalar coupling of a directly bonded 13C-13C pair has been used to create a double-quantum filter (DQF) to remove the natural-abundance 13C background in 13C{15N} rotational-echo double-resonance (REDOR) experiments. The DQF scalar and REDOR dipolar evolution periods are coincident which is important for sensitivity in the event of weak 13C-15N dipolar coupling. Calculated and observed 13C{15N} DQF-REDOR dephasings were in agreement for a test sample of mixed recrystallized labeled alanines. Glycine metabolism in a single uniform-15N soybean leaf labeled for 6 min by 13CO2 was measured quantitatively by 13C{15N} DQF-REDOR with no background interferences.

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AlgorithmsComputer SimulationGlycineMagnetic Resonance SpectroscopyModels, ChemicalPlant LeavesQuantum TheoryRotationGlycine maxSpin LabelsJournal ArticleResearch Support, Non-U.S. Gov't

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Shigeru Matsuoka, Jacob Schaefer. 2006-09-27. Double-quantum filtered rotational-echo double resonance.. https://doi.org/10.1016/j.jmr.2006.08.010

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