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PubMed · 17013382

Nonblinking and long-lasting single-molecule fluorescence imaging.

Ivan Rasnik·Sean A McKinney·Taekjip Ha
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Abstract

Photobleaching and blinking of fluorophores pose fundamental limitations on the information content of single-molecule fluorescence measurements. Photoinduced blinking of Cy5 has hampered many previous investigations using this popular fluorophore. Here we show that Trolox in combination with the enzymatic oxygen-scavenging system eliminates Cy5 blinking, dramatically reduces photobleaching and improves the signal linearity at high excitation rates, significantly extending the applicability of single-molecule fluorescence techniques.

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CarbocyaninesChromansFree Radical ScavengersMercaptoethanolMicroscopy, FluorescenceOxygenPhotobleachingSensitivity and SpecificityTime FactorsJournal ArticleResearch Support, N.I.H., Extramural

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Ivan Rasnik, Sean A McKinney, Taekjip Ha. 2006-10-01. Nonblinking and long-lasting single-molecule fluorescence imaging.. https://doi.org/10.1038/nmeth934

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